YAP (Yes-associated protein) dynamics in the Mouse Embryo transition phase based on fluorescence intensity data

In this modern era, the conversion of �images� into numerical data was very possible to do. In this study, we have transformed immunofluorescence images into intensity data by utilizing the Zen software. Three sample groups of in vitro cultured mouse embryo throughout the embryonic transition phase (8-cell, morula, and blastocyst) were used to identify the YAP (Yes-associated protein) expression. YAP allegedly was the most important protein in this differentiation process. The prepared samples were observed using the z-stack imaging method by using anLSM confocal. By this method, we are allowed to measure the dynamics of YAP on each cell. The study results showed that the total YAP expressed equally on each cell, but envoy a different manifestation. YAP discovered filling the nuclear, cytoplasmic, or both sides. Intensity analysis of each type of cell showed a non-significant difference among embryo group. In conclusion, cells maintained the YAP equilibrium during the embryonic transitional process. © 2021 Elsevier B.V., All rights reserved.