The effect of giving deltamethrin and cypermethrin insecticides on the level of intratesticular testosterone in male wistar strain rats

The use of deltamethrin and cypermethrin insecticides to control pests has been implemented in Indonesia. Irregular and excessive use of insecticides can have toxic effects on the male reproductive system. Deltamethrin and cypermethrin insecticides can inhibit androgen biosynthesis and disrupt the growth of sperm. Therefore, this research was conducted to determine the effect of deltamethrin and cypermethrin insecticides toward the level of intratesticular testosterone in male Wistar strain rats. This research was conducted in vivo using male Wistar strain rats. Nine rats were divided into three groups of treatment, namely control group, deltamethrin group with 0.26mg/kg of weight, and cypermethrin group with 0.26mg/kg of weight. Treatment was conducted every day for 15 days orally, and then the rats were dissected to take their testicles. Testicles were chopped and their intratesticular homogenates were taken by adding the medium of DMEM and collagenase (0.25mg/mL). The measurement of the level of testosterone was conducted by using the Electrochemiluminescence immunoassay (ECLIA) method. Data on the change of the increase of bodyweight, organ weight, and the level of testosterone was analyzed statistically using the one-way ANOVA test. The research result shows that giving 0.26mg/kg of weight of deltamethrin and 0.26mg/kg of weight of cypermethrin does not affect the increase in bodyweight, relative weight of the reproductive organ, and the level of intratesticular testosterone of male Wistar strain rats. © 2020 Elsevier B.V., All rights reserved.