The effect of cytotoxicity of collagen-chitosan hydrogel on platelets-rich plasma various formulation for human primary fibroblast

Introduction: Platelet-rich plasma (PRP) contains growth factors that accelerates the process of wound healing. The application of liquid PRP is tricky; therefore, it requires collagen to act as a carrier. Upon exposure to body fluids, collagen often loses its shape and size due to degradation. Collagen is synthesized with chitosan during material selection. The formulations of collagen-chitosan hydrogel in PRP that met the criteria of physical test were 25/75 and 0/100. However, to investigate the biocompatibility, MTT assay was employed to test cytotoxicity by detecting cell viability using human primary fibroblast. The aim of this study is to determine the cytotoxic effect of collagen-chitosan hydrogel formulation on PRP in human primary fibroblast. Methods: The study divided into three groups: Collagen-chitosan hydrogel in PRP 25/75 (n=9), collagen-chitosan hydrogel in PRP 0/100 (n=9), and PRP-only (n=9). After dripping MTT into the microplates, optical density were read using ELISA reader and the viability were counted. The data that was acquired from the viability calculation was analyzed with one-way ANOVA and LSD test. Results: There were significant differences (p=0.007) between collagen-chitosan hydrogel 25/75 on PRP and other groups. The collagen-chitosan hydrogel 25/75 group had the highest level than collagen-chitosan hydrogel 0/100 and PRP-only. Conclusion: This in vitro study demonstrated hydrogels collagen-chitosan ratio of 25/75 and 0/100 on PRP had no cytotoxic effects in human primary fibroblasts. © 2021 Elsevier B.V., All rights reserved.