Successful Artificial Insemination using Sexed and Non Sexed Semen on Limousin Crossbred Cow

Background: Beef cattle fattening business requires male cattle because they grow faster so that by using sexed semen (Y sperm) it is expected to get a male calf. The purpose of this study was to determine the success of artificial insemination using non-sexed and sexed frozen semen using percoll density gradient centrifugation (PDGC) method. Methods: A total of 120 female Limousin Crossbred were used in this study. The cows were raised on smallholder farms aged between 3-9 years with a body condition score (BCS) between 3-7 (score 1-9). The treatment was T0: 60 cows were inseminated using non-sexed frozen semen while T1: 60 cows were inseminated using sexed Y frozen semen. The insemination was performed with double doses at the 2nd and 8th hours after oestrus and deep insemination. The parameter measured were NRR-1, NRR-2, conception rate and pregnancy rates. Determination of pregnancy was performed using rectal palpation and ultrasonography (USG). Result: The results showed that NRR-1 and NRR-2 at T0 were 47 (78.33) and 42 (70), while at T1 were 37 (61.67) and 35 (58.33), respectively. While conception rate at T0 and T1 was 22 (36) and 18 (30), pregnancy rate at T0 and T1 was 31 (56) and 19 (31.67) and silent heat at T0 and T1 was 24 (40) and 26 (43.3). © 2025, Agricultural Research Communication Centre. All rights reserved.