The Influence of Hydration Temperature and Lipid Weight in C-4-hydroxy-phenylcalix[4]pyrogallolarene Liposome Preparation as Skin Brightening

The C-4-hydroxy-phenylcalix[4]pyrogallolarene is a compound proven to inhibit tyrosinase enzyme activity. This activity makes C-4-hydroxy-phenylcalix[4]pyrogallolarene as a potential skinbrightening agent. However, the poor water solubility of this compound limits its application as a skin brightener. Therefore, this study formulated C-4-hydroxy-phenylcalix[4]pyrogallolarene liposomes to address this issue. The liposomes were prepared using the thin-layer lipid film hydration method with L-αphosphatidylcholine as the lipid base. The resulting C-4-hydroxyphenylcalix[4]pyrogallolarene liposomes were found in spherical forms, and they exhibited multilamellar vesicle characteristics. This study also examined the influence of hydration temperature and L-α-phosphatidylcholine concentration on liposome size and polydispersity index (PDI). ANOVA analysis using Design Expert-13 indicated that the factors of hydration temperature, L-αphosphatidylcholine weight, and their interaction all influenced the size and PDI of the resulting C-4-hydroxy-phenylcalix[4] pyrogallolarene liposomes