The effect of feeding restriction on the microbiota and metabolome response in late-phase laying hens

Artdita, Clara Ajeng and Zhuang, Yi-Ru and Liu, Tzu-Yu and Cheng, Chih-Yuan and Hsiao, Felix Shih-Hsiang and Lin, Yuan-Yu (2021) The effect of feeding restriction on the microbiota and metabolome response in late-phase laying hens. Animals, 11 (11). ISSN 20762615

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Abstract

This study investigated cecal bacterial community profile, cecal and serum metabolites, and its biosynthesis pathway in late-phase laying hens during 6 weeks feeding restriction (FR), using 16S rDNA as gene sequencing and non-targeted LC-MS/MS as metabolomics approach. We used three groups (ad libitum, FR20, and FR40). FR can reduce excessive fat in late-phase laying hens, while egg production rate is not affected, except for the FR40 group. In phylum level, FR20 had more population of Bacteriodetes and Firmicutes amongst groups. The same result is at genus level, FR20 were higher of the predominant genus (Bacteroides and RikenellaceaeRC9gutgroup). Both of FR20 and FR40 reduced Proteobacteria as potential pathogenic bacteria. Non-targeted metabolomic analysis revealed that FR20 modified 20 metabolites in cecal and 10 metabolites in serum of laying hens, whereas 48 cecal metabolites and 31 serum metabolites has revealed in FR40. KEGG assay showed FR20 and FR40 upregulated lipid, carbohydrate, amino acid, nucleic acid pathway, and FR40 modified steroid metabolism in cecal analysis. In serum, only FR40 modified lipid, amino acid pathway, and carbohydrate biosynthesis were shown. This study showed that FR during late-phase laying hens altered the microbiome composition, modified metabolites profile and biosynthesis of the cecal as well as serum. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Item Type: Article
Additional Information: Cited by: 11; All Open Access, Gold Open Access, Green Open Access
Uncontrolled Keywords: amino acid; aspartic acid; carbohydrate; genomic DNA; lipid; pantothenic acid; pyridoxine; riboflavin; abdominal fat; animal experiment; animal model; Article; controlled study; diet restriction; DNA extraction; egg production; feeding; gel electrophoresis; liquid chromatography-mass spectrometry; metabolome; nonhuman; phylogeny; Proteobacteria; pyrosequencing; sequence analysis
Subjects: Veterinary Medicine
Divisions: Vocational School
Depositing User: Sri JUNANDI
Date Deposited: 04 Nov 2024 08:25
Last Modified: 04 Nov 2024 08:25
URI: https://ir.lib.ugm.ac.id/id/eprint/10508

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