Freeze-dried Human Platelet-rich Plasma Application Effect in Increase Alveolar Bone Height and Density of Periodontitis Rabbits

Introduction: Regenerative treatment with open flap debridement (OFD) and bone graft material containing growth factors is developed to repair alveolar bone defects due to periodontitis. Freeze-dried human platelet-rich plasma (FD-hPRP) is platelet-rich plasma which is freeze-dried to prolong storage time, simplify application, and increase growth factors number that promote osteoblast proliferation to accelerate bone regeneration. This study examined the effect of FD-hPRP application as a bone graft on the alveolar bone height and density radiographically in periodontitis treatment of Oryctolagus cuniculus rabbits. Methods: Twelve-male rabbits were periodontitis induced by ligation technique and Porphyromonas gingivalis lipopolysaccharide injection randomly divided into three groups: 1) OFD, 2) OFD+demineralized freeze-dried bone allograft (DFDBA), 3) OFD+FD-hPRP. OFD was performed in all groups with DFDBA applied in group 2 and FD-hPRP applied in group 3, then subjected to radiographic examination. The alveolar bone height data from Vet-Exam Plus and bone density data from ImageJ then analyzed with a two-way analysis of variance followed by Post Hoc Least Significant Difference test. Results: Significant difference (p<0.05) between the group 1 and group 2 and 3, and an insignificant difference (p>0.05) between the group 2 and 3. Conclusion: FD-hPRP application increased alveolar bone height and density radiographically in periodontitis rabbits. © 2023 UPM Press. All rights reserved.