Effect of Citrus Sinensis Peel Extract Gel on Periodontal Healing in Rat Model

Introduction: Periodontitis is chronic inflammatory disease which could lead to the loss of teeth. Citrus sinensis peel extract gel is a potent host modulating therapy due to its rich flavonoid contents. The purpose of this study is to determine the efficacy of a 10 C. sinensis peel extract gel application following curettage. In periodontal disease healing in rat model. Methods: The lower incisor of 24 Sprague Dawley rat were ligated to induce periodontitis. The negative control group received 2 CMC-Na, treatment group received 10 C. sinensis peel extract gel, and the positive control group received Gengigel® gel following curettage. At days 3, 5, 7, and 14, two rats from each group were euthanized. Histological examination was conducted to assess angiogenesis, collagen density, fibroblast, and osteoclast count. Two-way ANOVA and the Post Hoc test were used to analyze the data. Results: Angiogenesis and fibroblast count were statistically significantly different in the treatment group (p<0,05) among the negative, treatment and positive control groups at day 3, 5, 7, and 14. Collagen density on treatment group was statistically different (p<0,05) between negative, control, and treatment group at day 7 and 14. At days 3 and 7, the osteoclast count in the treatment group was statistically different from that in the negative control group. Conclusion: Application of 10 C.sinensis peel extract gel following curettage increased angiogenesis, fibroblast count, collagen density, and decreased osteoclast count in periodontal tissue in rat models. © 2023 UPM Press. All rights reserved.