Effect of Freeze-dried Homologous Platelet-rich Plasma Preparation and Sterilization Methods on Levels TGF-β

Introduction: Studies showed that Homologous platelet-rich plasma (H-PRP) is superior to Autologous PRP (A-PRP) which contained larger amount of Transforming growth factor-β (TGF-β) which applicable to numbers of clinical application, but PRP in general had a disadvantage of a short shelf life and impracticality . Our study uses H-PRP, as a by-product from transfusion processes conducted in the Indonesian Red Cross (PMI). The H-PRP had passed transfusion-related infection screening and were rarely used. Sedimentation (S) and single centrifugation (SC) methods were used to produce H-PRP. The H-PRP were freeze-dried (FD) to maintain the Transforming growth factor-β (TGF-β) content more durable and made it easier to apply. FD-H-PRP underwent γ-irradiation Sterilization process. TGF-β plays an important role in the wound healing process and is the most abundant growth factor in platelets. This study explored the effects of FD and γ-irradiation sterilized H-PRP on TGF-β1 levels. Methods: Total of 40 FD-H-PRP samples were used, which were divided into 10 FD-H-PRP(S), 10 FD-H-PRP(S), 10 FD-H-PRP(SC), and 10 FD-H-PRP(SC) that were exposed to 20, and 25 KGy γ-irradiation doses, respectively. Enzyme-linked immunoassay test were used to measure TGF-β levels. A nonparametric test was used for statistical analyses. Results: TGF-β levels are significantly higher in H-PRP samples generated using the S method than those using the SC method and in samples with a γ-irradiation dose of 25-KGy than those with a 20-KGy γ-irradiation dose. Conclusion: Lowercentrifugation force increases the platelet count and its growth factors, which proved that the S method is superior to the SC method. In contrast, higher γ-irradiation dose administered to platelets produce higher TGF-β levels. © 2023 UPM Press. All rights reserved.