Sakti, A.A. and Suwignyo, B. and Sofyan, A. and Hanim, C. and Herdian, H. and Jasmadi, J. and Pasaribu, T. and Julendra, H. and Gunawan, G. and Ratnawati, P. and Hartati, L. and Tarigan, S.A.E. and Adiwimarta, K. (2024) In vitro anthelmintic activity of Chaetomorpha vieillardii ethanolic extract against adult worm motility and egg-hatching of Haemonchus contortus from sheep. Veterinary Integrative Sciences, 22 (2). pp. 475-487. ISSN 26299968
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Abstract
This study aimed to evaluate the potential of macroalgae Chaetomorpha vieillardii as an in vitro candidate for bio-anthelmintic. Adult Worm Motility Test (AWMT) was conducted at concentration levels of 2 mg/mL, 4 mg/mL, and 6 mg/mL of C. vieillardii ethanol extract on 150 female H. contortus worms, randomly divided into 6 treatments with 5 replications, using a two way completely randomized factorial design. 0.9 sodium chloride was used as a negative control, and albendazole at 0.5 and 2 mg/mL served as positive controls. The observation of worm motility was conducted at time intervals of 0, 0.25, 0.5, 1, and 2 hours, and subsequently at hourly intervals until 100 of the worms were deceased. The identical treatment was applied to the Egg Hatching Inhibiting Test (EHIT) using a completely randomized design in a one-way pattern by observing the number of eggs at 0 and 24 hours. The results showed that the concentration level of C. vieillardii ethanol extract and the observation time significantly influenced (P<0.01) the motility of H. contortus, and there was an interaction between them (P<0.01). All three levels were able to decrease the motility of H. contortus, but they were unable to match albendazole. Despite this result, the three levels were equally effective (P<0.01) as albendazole in terms of inhibiting the egg-hatching of H. contortus. The 2 mg/mL level was sufficient to inhibit the motility and egg-hatching of H. contortus. These findings indicated that the ethanol extract of C. vieillardii has the potential to be developed as a bio-anthelmintic for ruminants. © 2021 Author (s).
Item Type: | Article |
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Additional Information: | cited By 0 |
Subjects: | S Agriculture > SF Animal culture T Technology > TS Manufactures > Animal products |
Divisions: | Faculty of Animal Sciences > Department of Animal Nutrition and Feed Science |
Depositing User: | Arif Surachman |
Date Deposited: | 01 Oct 2024 00:45 |
Last Modified: | 01 Oct 2024 00:45 |
URI: | https://ir.lib.ugm.ac.id/id/eprint/11 |