Enam expression is regulated by Msx2

Ruspita, Intan and Das, Pragnya and Miyoshi, Keiko and Noma, Takafumi and Snead, Malcolm L. and Bei, Marianna (2023) Enam expression is regulated by Msx2. Developmental Dynamics, 252 (10). 1292 – 1302. ISSN 10588388

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Abstract

Background: The precise formation of mineralized dental tissues such as enamel and/or dentin require tight transcriptional control of the secretion of matrix proteins. Here, we have investigated the transcriptional regulation of the second most prominent enamel matrix protein, enamelin, and its regulation through the major odontogenic transcription factor, MSX2. Results: Using in vitro and in vivo approaches, we identified that (a) Enam expression is reduced in the Msx2 mouse mutant pre-secretory and secretory ameloblasts, (b) Enam is an early response gene whose expression is under the control of Msx2, (c) Msx2 binds to Enam promoter in vitro, suggesting that enam is a direct target for Msx2 and that (d) Msx2 alone represses Enam gene expression. Conclusions: Collectively, these results illustrate that Enam gene expression is controlled by Msx2 in a spatio-temporal manner. They also suggest that Msx2 may interact with other transcription factors to control spatial and temporal expression of Enam and hence amelogenesis and enamel biomineralization. © 2023 American Association for Anatomy.

Item Type: Article
Additional Information: Library Dosen
Uncontrolled Keywords: Ameloblasts; Animals; Gene Expression Regulation; Mice; Odontogenesis; Promoter Regions, Genetic; Transcription Factors; enamel protein; enamelin; transcription factor MSX2; unclassified drug; transcription factor; ameloblast; amelogenesis; animal cell; animal experiment; animal tissue; Article; binding site; controlled study; early response gene; enam gene; gene expression; gene repression; gene targeting; in vitro study; in vivo study; mouse; mouse mutant; nonhuman; promoter region; protein binding; spatiotemporal analysis; tooth development; transcription regulation; animal; gene expression regulation; metabolism; tooth development
Subjects: R Medicine > RK Dentistry
Depositing User: Sri JUNANDI
Date Deposited: 07 Nov 2024 05:59
Last Modified: 07 Nov 2024 05:59
URI: https://ir.lib.ugm.ac.id/id/eprint/11004

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