The Effect of Carbonated Hidroxyapatite-10 Propolis Application on Open Flap Debridement Towards Transforming Growth Factor Β1 Expression

Introduction: Carbonated Hydroxyapatite (CHA) is used as a bone graft material in the treatment of periodontitis in dentistry. Carbonated Hydroxyapatite has high biocompatibility, osteoconductive, and as a drug carrier. Propolic is a resinous material from bees that has antibacterial, anti-inflammatory, and accelerates wound healing. The incorporation of CHA with propolic is expected to accelerate alveolar bone regeneration better by increasing the expression of Transforming Growth Factor β1 in the treatment of periodontitis. This study’s aim was to examine the effect of CHA-Propolic 10 application on open flap debridement (OFD) and TGF-β1 expression. Methods: This study use 24 sample from six male rabbits. The fourth tissue slices periodontitis from each treatment group was taken from the mandibular incisors of the rabbit Oryctolagus Cuniculus that had been induced by ligation and injected with LPS of Propyromonas gingivalis bacteria. Periodontitis conditions in rabbits were obtained after 2 weeks of induction of periodontitis then the rabbits were treated and divided into 3 groups, group I only OFD action, group II OFD action added CHA application, and group III OFD action added 10 Propolic-CHA application. Decapitation was done on the 7th day and 4th day. The data were analyzed using the Shapiro-Wilk test, Anava two paths test, and continued with the Least Significant Difference (LSD) test. Results: The results showed CHA incorporated with a 10 propolic group had a significant difference compared to other treatment groups (p<0,05). Conclusion: The conclusion obtained from this study was that the application CHA incorporated with a 10 propolic group shows the ability to increase the expression of TGF-β1 alveolar bone of rabbits. © 2023 UPM Press. All rights reserved.