Remodeling in early myocardial infarction: alteration of extracellular matrix; Collagen-1, Collagen-3, α-SMA, and α-Actinin in Porcine heart model

Putra, Muhammad Arza and Alwi, Idrus and Soetisna, Tri Wisesa and Gunanti, Gunanti and Sandora, Normalina and Busro, Pribadi Wiranda and Supomo, Supomo and Fitria, Nur Amalina and Kusuma, Tyas Rahmah (2023) Remodeling in early myocardial infarction: alteration of extracellular matrix; Collagen-1, Collagen-3, α-SMA, and α-Actinin in Porcine heart model. Bali Medical Journal, 12 (3). 2721 – 2728. ISSN 20891180

[thumbnail of Remodeling in early myocardial infarction.pdf] Text
Remodeling in early myocardial infarction.pdf
Restricted to Registered users only

Download (849kB)

Abstract

Background: Myocardial infarction (MI) leads to remodeling due to altered cardiac structure and function. The study of MI histopathology in humans or large animals mimicking the human heart is limited. Using the porcine heart model, our study investigated the composition of myocardial extracellular matrix (ECM) in collagen-1, collagen-3, α-smooth muscle actin (α-SMA), and α-actinin before and after myocardial infarction. Methods: This study used two groups of domestic pigs: the Infarct group (n=4) and the Sham group (n=4). MI was induced by permanent ligation of the proximal branch of the posterior left ventricular artery. Cardiac enzymes, electrocardiography, and echocardiography data were collected before and after ligation. Cardiac tissue was harvested from the infarcted area after 60 minutes of ligation and stained with hematoxylin-eosin and Movat’s Pentachrome. Collagen-1, collagen-3, and α-SMA were identified with immunohistochemical labeling, and the labeled area was measured using ImageJ. Meanwhile, α-actinin was visualized using immunofluorescence. Results: Expression of collagen-1, collagen-3, and α-SMA in the infarct group were significantly decreased after 60 minutes of infarction compared with those in the sham group (p<0.01). The α-actinin was fragmented and diminished in the infarct group. Conclusion: Myocardial remodeling was detected 60 minutes after infarction with mild alteration in myocardial histoarchitecture and significant deterioration of ECM composition of collagen-1, collagen-3, α-SMA, and fragmented α-actinin fibers in the porcine heart model. © 2023, Sanglah General Hospital. All rights reserved.

Item Type: Article
Additional Information: Cited by: 1; All Open Access, Gold Open Access
Subjects: R Medicine > RC Internal medicine
Depositing User: Annisa Fitria Nur Azizah Annisa Fitria Nur Azizah
Date Deposited: 16 May 2024 01:56
Last Modified: 16 May 2024 01:56
URI: https://ir.lib.ugm.ac.id/id/eprint/1105

Actions (login required)

View Item
View Item