Rizal, Dicky Moch and Setiawan, Jajar and Susanti, Susi and Adzillina, Laksmita Dewi and Septiyorini, Nandia (2023) Hyperglycemia may induce a decrease in myoblast viability: A Preliminary Study. Bali Medical Journal, 12 (3). pp. 2715-2720. ISSN 20891180
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Abstract
Background: Hyperglycemia plays a significant role in developing insulin resistance, leading to a continuous decline in metabolism. Uncontrolled long-term hyperglycemia has deleterious effects on muscles, leading to a decline in muscle mass, impaired capillarization, and muscle atrophy. We identified the effect of hyperglycemia on reducing myoblast cell viability through observation using the MTT assay. Methods: Primary culture of myoblast cells was performed using rat’s muscle tissue which was further induced by hyperglycemia by adding D-glucose with concentrations of 10 mM, 25 mM, and 50 mM in the plating media. Cell viability was observed using the MTT assay by identifying the absorbance using an ELISA reader with λ=550-600 nm (595 nm). Results: There was a significant decrease in cell viability (p=0.000) due to administering various glucose concentrations in the plating media. There is a negative correlation between glucose concentration and myoblast cell viability (P-value = 0.000; t =-8.284; r =-0.926). Conclusion: Elevated blood sugar levels result in a significant reduction in the viability of myoblast cells, as indicated by the MTT assay. The MTT assay proved invaluable for precisely evaluating myoblast cell viability in the context of hyperglycemia. © 2023, Sanglah General Hospital. All rights reserved.
Item Type: | Article |
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Additional Information: | Cited by: 0; All Open Access, Gold Open Access |
Uncontrolled Keywords: | Cell Toxicity, Hyperglycemia, MTT Assay, Myoblast Cell Viability |
Subjects: | R Medicine > R Medicine (General) |
Divisions: | Faculty of Medicine, Public Health and Nursing > Biomedical Sciences |
Depositing User: | Ani PURWANDARI |
Date Deposited: | 05 Jun 2024 06:17 |
Last Modified: | 05 Jun 2024 06:17 |
URI: | https://ir.lib.ugm.ac.id/id/eprint/1118 |