Combination Effect of Rotator Cuff Repair with Secretome-hypoxia MSCs Ameliorates TNMD, RUNX2, and Healing Histology Score in Rotator Cuff Tear Rats

Fredianto, Meiky and Herman, Herry and Dias Ismiarto, Yoyos and Purba, Ambrosius and Putra, Agung and Hidayah, Nurul (2023) Combination Effect of Rotator Cuff Repair with Secretome-hypoxia MSCs Ameliorates TNMD, RUNX2, and Healing Histology Score in Rotator Cuff Tear Rats. Archives of Bone and Joint Surgery, 11 (10). 617 -624. ISSN 23454644

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Abstract

Objectives: In order to treat a rat model of rotator cuff rupture, this work concentrated on the expression of TNMD and RUNX2, followed by rotator cuff repair and secretome-hMSCs. Methods: A total of thirty 10-weeks-old male Sprague–Dawley rats were separated into five groups randomly, RC on week 0, lesion treated with a rotator cuff repair and saline (RC + NaCl group, n = 6) for 2 and 8 weeks, and lesion treated with a rotator cuff repair and secretome-hMSCs (RC + secretome-hMSC group, n = 6) for 2 and 8 weeks. The supraspinatus and infraspinatus muscle–tendon units were obtained for histological and biomechanical investigation at 0, 2 and 8 weeks following injury. Results: The findings showed that, in comparison with the RC + NaCl group, secretome-hMSCs significantly improved tendon repair by upregulating TNMD and RUNX2 expression and histology score. Conclusion: Combining Secretome-hypoxia MSCs with RC healing may help rats with rotator cuff tears. © 2023 Mashhad University of Medical Sciences. All rights reserved.

Item Type: Article
Additional Information: Cited by: 0
Uncontrolled Keywords: sodium chloride; transcription factor RUNX2; adult; animal cell; animal experiment; animal model; animal tissue; Article; biomechanics; cell hypoxia; controlled study; histology; infraspinatus muscle; male; mesenchymal stem cell; nonhuman; protein expression; rat; rat model; rotator cuff repair; rotator cuff rupture; secretome
Subjects: R Medicine > RZ Other systems of medicine
Divisions: Faculty of Medicine, Public Health and Nursing > Nursing
Depositing User: Ani PURWANDARI
Date Deposited: 27 May 2024 08:38
Last Modified: 27 May 2024 08:38
URI: https://ir.lib.ugm.ac.id/id/eprint/1153

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