Effects of Caffeine Mixed with Alpha Lipoic Acid in Preventing Streptozotocin-induced Diabetes in Rats: In Silico and In vivo Study

Abidin, Khoirul Rista and Meliala, Andreanyta and Rini, Sri Lestari Sulistyo (2023) Effects of Caffeine Mixed with Alpha Lipoic Acid in Preventing Streptozotocin-induced Diabetes in Rats: In Silico and In vivo Study. Journal of Health Science and Medical Research, 41 (6). ISSN 25869981

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Abstract

Objective: This study aimed to investigate the combined effects of caffeine and Alpha Lipoic Acid (ALA) on oxidative stress, due to chronic hyperglycemia, in a model of diabetic rats induced with streptozotocin (STZ) (in silico and in vivo approaches). Material and Methods: This In silico study investigated the interaction between caffeine and ALA against insulin receptors and enzymes of Glutathione Peroksidase-1 (GPx-1), with molecular docking. Male, Wistar rats were included using a quasi-experimental research design, with post-test only and a control group (in vivo). This study measured the end result of a 6-week-induction on body weight, fasting blood glucose, Malondialdehyde (MDA) and GPx-1 enzyme from 25 rats. Results: Molecular docking found the interactions of caffeine and GPx-1 consisting of an energy bond of-5,06 kcal/mol, hydrogen and hydrophobic bond. Additionally, it showed the interaction of ALA and GPx-1 containing an energy bond of-5.16 kcal/mol, hydrogen bonding and hydrophobicity. However, there were no significant difference in body weight, fasting blood glucose, MDA and GPx-1 levels of the ALA-caffeinated diabetic rats compared to diabetic rats. Conclusion: Caffeine and ALA have the potential to activate GPx-1 enzymes (in silico study). However, the use of a caffeine and ALA combination resulted in no significant difference in fasting blood glucose and oxidative stress conditions when compared to diabetic rats without additional induction (in vivo study). © 2023 JHSMR. Hosted by Prince of Songkla University. All rights reserved.

Item Type: Article
Additional Information: Cited by: 0; All Open Access, Gold Open Access
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Medicine, Public Health and Nursing > Biomedical Sciences
Depositing User: Annisa Fitria Nur Azizah Annisa Fitria Nur Azizah
Date Deposited: 16 May 2024 01:26
Last Modified: 16 May 2024 01:26
URI: https://ir.lib.ugm.ac.id/id/eprint/1200

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