Phenotypic characteristic of Staphylococcus aureus from subclinical mastitis in Etawah-crossbreed goats in Yogyakarta, Indonesia

Suwito, Widodo and Nugroho, Widagdo Sri and Adji, Rahmat Setya and Andriani, Andriani and Kusumaningtyas, Eny and Martini, Tri (2022) Phenotypic characteristic of Staphylococcus aureus from subclinical mastitis in Etawah-crossbreed goats in Yogyakarta, Indonesia. Veterinary World, 15 (11). pp. 2587-2592. ISSN 09728988

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Abstract

Background and Aim: Subclinical mastitis (SCM) in Etawah-grade (PE) goats in Yogyakarta, Indonesia, is commonly due to Staphylococcus aureus. At present, S. aureus from SCM in PE goats in Yogyakarta has not been characterized.
Therefore, this study aimed to phenotypically characterize S. aureus, which has been isolated from SCM of PE goats.

Materials and Methods: A total of 314 lactating PE goats were collected from 60 PE goat farms (e.g., Sleman, Bantul, and Kulonprogo) located in parts of Yogyakarta with an average age of 3–4 years old, three of which showed SCM based on the California mastitis test (CMT). Subclinical mastitis is confirmed in PE goats if CMT shows ++ or +++. Furthermore, S. aureus was detected by biochemical assays. Staphylococcus aureus could determine hemolysin (Hae), coagulase (Coa), clumping factor (Cf), and antibiotic susceptibility. Hemolytic bacteria were detected by culturing on blood agar plate, and Cf was detected by slide agglutination. The production of Coa was
detected by tube coagulation. Staphylococcus aureus susceptibility was determined by antimicrobial agar diffusion
using a paper disc.

Results: Phenotypically characterized S. aureus from PE goats with SCM in Yogyakarta, Indonesia, Coa−, Cf−, and Hae−
were found to be resistant to erythromycin (ERYTHRO), ampicillin (AMP), penicillin (PEN-G), and sulfamethoxazole
(SULFA).

Conclusion: The phenotypic characteristic of S. aureus, which was obtained from SCM in PE goats in Yogyakarta, consists
of Coa, and Cf−. S. aureus cannot perform hemolysis of red blood cells. This phenotypic characteristic can prevent and
control SCM in PE goats. Several antibiotics such as ERYTHRO, AMP, PEN-G, and SULFA were no longer effective for
treating SCM in PE goats because S. aureus has developed its resistance to these antibiotics.

Item Type: Article
Additional Information: Library Dosen
Uncontrolled Keywords: Etawah-grade goats, phenotypic, Staphylococcus aureus, subclinical mastitis
Subjects: Veterinary Medicine
Divisions: Faculty of Veterinary Medicine
Depositing User: Erlita Cahyaningtyas Cahyaningtyas
Date Deposited: 10 Dec 2024 01:01
Last Modified: 10 Dec 2024 01:01
URI: https://ir.lib.ugm.ac.id/id/eprint/12207

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