Design and Performance Evaluation of Alternative Home Care Systems for Detecting SARS CoV-2 using the RT-LAMP Method

The global pandemic announced by the World Health Organization (WHO) in January 2020 has infected many people in various countries. The Covid-19 pandemic, caused by SARS CoV-2, is a disease that is difficult to diagnose because of its non-specific clinical symptoms. Some observed clinical symptoms include fever, cough, or shortness of breath that occurs approximately two days after exposure to the virus. RT-qPCR is a method that can be used to detect the SARS CoV-2 virus, with high sensitivity and specificity. However, this method has several disadvantages, including requiring special equipment, a long process, high costs, and the need for special expertise from operators and analysts in implementing the method. RT-LAMP is an alternative method that can detect the SARS CoV-2 virus faster with high sensitivity. The advantages of RT-LAMP compared to the RT-qPCR method are that it requires less equipment, a relatively shorter time, and a lower cost to detect the SARS CoV-2 virus. Our current study reported that the RT-LAMP machine showed an accuracy of 96.67 in detecting the presence of the SARS CoV-2 virus. © 2024 American Institute of Physics Inc.. All rights reserved.