In Vivo Regulation of Active Matrix Metalloproteinase-8 (aMMP-8) in Periodontitis: From Transcriptomics to Real-Time Online Diagnostics and Treatment Monitoring

Aji, Nur Rahman Ahmad Seno and Yucel-Lindberg, Tuelay and Raeisaenen, Ismo T. and Kuula, Heidi and Nieminen, Mikko T. and Mc Crudden, Maeliosa T. C. and Listyarifah, Dyah and Lundmark, Anna and Lundy, Fionnuala T. and Gupta, Shipra and Sorsa, Timo (2024) In Vivo Regulation of Active Matrix Metalloproteinase-8 (aMMP-8) in Periodontitis: From Transcriptomics to Real-Time Online Diagnostics and Treatment Monitoring. DIAGNOSTICS, 14 (10). pp. 1-14. ISSN 2075-4418

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Abstract

Background: This study investigated in vivo regulation and levels of active matrix metallop roteinase-8 (aMMP-8), a major collagenolytic protease, in periodontitis. Methods: Twenty-seven adults with chronic periodontitis (CP) and 30 periodontally healthy controls (HC) were enrolled
in immunohistochemistry and transcriptomics analytics in order to assess Treponema denticola (Td) dentilisin and MMP-8 immunoexpression, mRNA expression of MMP-8 and its regulators (IL-1β, MMP-2, MMP-7, TIMP-1). Furthermore, the periodontal anti-infective treatment effect was monitored
by four different MMP-8 assays (aMMP-8-IFMA, aMMP-8-Oralyzer, MMP-8-activity [RFU/minute], and total MMP-8 by ELISA) among 12 CP (compared to 25 HC). Results: Immunohistochemistry revealed significantly more Td-dentilisin and MMP-8 immunoreactivities in CP vs. HC. Transcriptomics revealed significantly elevated IL-1β and MMP-7 RNA expressions, and MMP-2 RNA was slightly reduced. No significant differences were recorded in the relatively low or barel.

Item Type: Article
Uncontrolled Keywords: active matrix metalloproteinase-8; aMMP-8; Td-dentilisin; periodontitis; transcriptomic; proteomic
Subjects: R Medicine > RK Dentistry
Divisions: Faculty of Dentistry > Dental Study Program Academic Phase
Depositing User: Desy Natalia Anggorowati Anggorowati
Date Deposited: 14 Feb 2025 08:41
Last Modified: 14 Feb 2025 08:41
URI: https://ir.lib.ugm.ac.id/id/eprint/13901

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