High Fiber Diets Enhance <i>IL</i>-<i>10</i> Gene Expression and Itslevelin Hyperlipidemic Rats Model

Hyperlipidemia induces inflammation by increasing proinflammatory cytokines and reducing anti-inflammatory cytokines. Short chain fatty acids from fiber fermented by intestinal bacteria can reduce inflammation. The aim of this study was to evaluate the benefits of high fiber diet on IL-10 gene expression and its levels in white adipose tissue in rats with high fat and fructose diet. High fructose and trans-fat were used to induce hyperlipidemia, while intervention diet for treatment 1 (HL1), treatment 2 (HL2), and treatment 3 (HL3) contain a total fiber of 6.88%, 13.77% and 20.65%, respectively. Serum IL-10 level was measured using ELISA method before and after intervention, and IL-10 gene expression was analyzed using quantitative PCR (qPCR) after intervention. Results: High trans-fat and fructose diet decrease IL-10 levels, while high-fiber diet can significantly increase the gene expression and levels of IL-10 in hypertriglyceridemia rats (p<0.05). The increased IL-10 levels was 58.36 pg/mL, 55.86 pg/mL, 76.70 pg/mL respectively within diet containing fiber for 6.88 %, 13.77 %, and 20.65%. However, only diet with 20.65% fiber could significantly increase IL-10 gene expression (1.26 + 0.54 become 2.74. + 0.22). Conclusion: High fiber diet could decrease inflammation through increase the gene expression and levels of IL-10.