Effect of Platelet-Rich Plasma (PRP) on testicular damage in streptozotocin-induced diabetic rats

Background: Diabetes mellitus (DM) is a condition which could lead to induce testicular damage due to oxidative stress by hyperglycemic stress. Several therapies have been elucidated to reduce the testicular damage; one of them is Platelet-Rich Plasma (PRP). PRP has known growth factor properties which may serve potential effects in the tissue regeneration. This study aims to determine the role of PRP on testes in streptozotocin-induced diabetic rats by evaluating its impact on testosterone serum level. Methods: A randomized clinical trial with control group design has been conducted among 20 rats in this study. The sample of rats was divided into four groups such as control, DM (streptozotocininduced), DM-Ins (treated with insulin daily), and DM-Ins-PRP (treated with insulin daily and PRP thrice in 2 weeks). Six weeks later, the testosterone level was measured by ELISA, and the right testis was collected for histopathology findings. Data were analyzed using SPSS version 25 for Windows. Results: The testes weight in the control group was 1.61 +/- 0.37 grams, followed by DM group (0.86 +/- 0.22 grams), DMIns group (1.15 +/- 0.25 grams), and DM-Ins-PRP group (1.09 +/- 0.36 grams). DM reduced testicular weight, seminiferous tubules diameter, the thickness of the epithelium and testosterone serum level significantly (p< 0.05) compared with control. PRP administration increases the thickness of seminiferous epithelium (p<0.05) significantly. Besides, it also increased the testicular weight, tubules diameter, lumen diameter, and testosterone serum level, but not significant (p>0.05). Conclusion: The administration of PRP showed a significant role in repairing testicular damage among diabetic rats.