The effect of vancomycin on the growth and microbial dynamics of microalgae-bacteria consortium isolated from Glagah Beach, Yogyakarta

The interaction between microalgae and bacteria in aquaculture might hold the key to the increase of biomass production from microalgae culture. With at least 22 phyla having been identified from the phycosphere, bacteria are known for being able to produce essential molecules for microalgae in a consortium culture. A consortium of microalgae and bacteria isolated from Glagah Beach, Yogyakarta Indonesia, named Glagah Isolate, showed to have higher biomass of that a single species cultivation. The effect of Vancomycin antibiotic on bacterial composition and diversity of the consortium culture was investigated using metabarcoding approach to compare between the treated (group A) and untreated samples (group T). The region V6 in the 16S rRNA gene was sequenced by using the MiSeq platform at 2×301 PE, and the library of V6 was eventually pooled according to the Illumina protocol. There were 14 phyla found in the consortium culture where was dominated by phylum Proteobacteria since it had the highest relative abundance on the lag, log, and stationary phase of the group T and the group A. The group T had higher diversity than the group A. The result from the growth imply that bacterial community has dynamically changed due to antibiotic treatment and affect the growth of microalgae. © EM International.