DMSO and sucrose combination as a cryoprotectant of Najawa carp (Cyprinus carpio L.) sperm cryopreservation

This study was designed to evaluate a DMSO (Dimethyl Sulfoxide) and sucrose combination as a cryoprotectant for cryopreservation of Najawa carp (Cyprinus carpio L.) sperm. The experiment was conducted according to the Completely Randomized Design (CRD) with four treatments 10 DMSO + 0.2 M sucrose, 10 DMSO + 0.1 M sucrose, 5 DMSO + 0.2 M sucrose, 5 DMSO + 0.1 M sucrose. Each treatment consisted of three replications. The parameter observed were sperm progressive motility of fresh sperm, diluted sperm before freezing, and diluted sperm after freezing 1 day; 7 days; 14 days; 21 days; 28 days. The data were analyzed by ANOVA. The result showed that there was no significant difference between treatments (Sig. > 0.05). The highest relative protection was 89.51 carried out by 5 DMSO + 0.1 M sucrose. © The Authors, published by EDP Sciences, 2020.