Molecular detection of toxoplasmosis in wild rats using loop-mediated isothermal amplification assay

Puspitasari, Heni and Suwanti, Lucia Tri and Mufasirin, Mufasirin and Kusnoto, Kusnoto and Yudaniayanti, Ira Sari and Setiawan, Boedi and Suprihati, Endang and Aksono, Eduardus Bimo and Widodo, Dwi Priyo and Wardhana, April Hari and Matsubayashi, Makoto and Indasari, Elly Nur (2024) Molecular detection of toxoplasmosis in wild rats using loop-mediated isothermal amplification assay. Veterinary World, 17 (7). pp. 1575-1580. ISSN 09728988

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Abstract

Background and Aim: Toxoplasmosis is caused by the parasite Toxoplasma gondii. Cats are the only known hosts that excrete resistant oocysts. Wild rats serve as crucial reservoirs and intermediate hosts for T. gondii’s survival and dissemination. Consuming soil and water containing oocysts can lead to illness. This study aimed to estimate the prevalence of toxoplasmosis in wild rats through molecular detection as an indicator of environmental contamination in Surabaya. Materials and Methods: One hundred rats were collected from the three areas (housing, dense settlements, and traditional markets) and distributed into the five zones: West, East, Central, North, and South of Surabaya. Brain tissue samples were extracted using a Geneaid™ (New Taipei City, Taiwan) DNA isolation kit and analyzed through the loop-mediated isothermal amplification (LAMP) method. Results: The study analyzed brain tissue from 100 wild rats, consisting of 77 Rattus tanezumi and 33 Rattus norvegicus, displaying 30% LAMP positivity. The study revealed that 30% (30/100) of wild rats tested were infected with T. gondii. The molecular prevalence rate in male rats was 32.35% (22/68), compared to females with 25% (8/32). 41.9% of the housing population, 33.3% of traditional markets, and 22.6% of dense settlements had the highest molecular prevalence. The high positive molecular rate at the trapping site can be attributed to cats and dense populations. Conclusion: Thirty percentage wild rats were tested positive for toxoplasmosis in Surabaya, East Java, Indonesia using LAMP method. Implementing strict control and monitoring is crucial in preventing the transmission of diseases from wild rats to humans. It is necessary to carry out further research related to genetic analysis of T. gondii to determine the type of T. gondii that infects animals and humans in Surabaya through bioassay and molecular test.

Item Type: Article
Uncontrolled Keywords: loop-mediated isothermal amplification, public health, tissue cyst, toxoplasmosis, wild rats
Subjects: Veterinary Medicine
Divisions: Faculty of Veterinary Medicine
Depositing User: Erlita Cahyaningtyas Cahyaningtyas
Date Deposited: 20 Mar 2025 03:54
Last Modified: 20 Mar 2025 03:54
URI: https://ir.lib.ugm.ac.id/id/eprint/15830

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