Silver Nanoparticles as an Antibacterial Candidate for Poultry: An Alternative to Synthetic Antibiotics

This study aimed to determine the concentration of silver nanoparticles that can inhibit gram-positive and negative bacteria. It was done so that the silver nanoparticles could be evaluated as an antimicrobial agent, and there was also the possibility that they could be used as a feed additive. This study assesses microbial inhibition and the minimum inhibitory concentration (MIC) in a laboratory setting. The substances utilized included silver nanoparticles, nutrient agar medium, Man’s Rogosa Sharpe Agar, Man’s Rogosa Sharpe Broth, bacterial cultures of Lactobacillus acidophilus ATCC 4356, and Lactobacillus sp. FNCC 0020, Salmonella typhimurium ATCC 700720, Escherichia coli FNCC 0091, tetracycline, and 70% alcohol. Antibiotic sensitivity testing employed the Kirby-Bauer and optical density 600 techniques to assess microbial growth inhibition and the minimum inhibitory concentration (MIC). The research data was examined for comparative statistics using a completely randomized design. Concentrations between 10 and 50 ppm in the inhibition zone test successfully inhibited the growth of Salmonella typhimurium and Escherichia coli while not affecting the viability of Lactobacillus acidophilus, Lactobacillus sp. The MIC for gram-negative bacteria such as Salmonella typhimurium and Escherichia coli was 6.25 ppm, and the optical density at 600 was approximately 0.09.