The Potential of Blue Laser to Activate Green Dye Photosensitizer Agents for Increasing Microbial Deaths in Biofilm and Planktonic Forms through Photoinactivation Mechanisms

Some medicinal plants with high chlorophyll content and numerous antibacterial substances have proven effective in killing any microbe. Noni leaf (Morinda citrifolia L.) and Castor leaf (Jatropha curcas L.). This study used two pathogenic microbial cultures, Streptococcus mutans planktonic and Staphylococcus epidermidis biofilm. This study aims to determine the potential of noni leaf extract to reduce cells of Streptococcus mutans, while castor leaf extract is applied to minimize Staphylococcus epidermidis. The optical density (OD) value after dyeing the XTT assay by combining groups with or without radiation will be determined quantitatively of their potential for photoinactivation mechanisms. This parameter is a characteristic and profile of free radical compounds produced by chlorophyll extract on photoactivation. The results showed that blue light could inactivate the planktonic cell more than biofilm. The decrease in absorbance intensity of the chlorophyll from both extracts after irradiance showed the radical compound activity. It has a high potential for use in photoinactivation mechanisms.