High Fiber Diets Enhance IL-10 Gene Expression and Itslevelin Hyperlipidemic Rats Model

Hyperlipidemia induces inflammation by increasing proinflammatory cytokines and reducing anti-inflammatory cytokines. Short chain fatty acids from fiber fermented by intestinal bacteria can reduce inflammation. The aim of this study is to evaluate the benefits of high fiber diet on IL-10 gene expression and IL-10 levels in white adipose tissue in rats with high fat and fructose diet. Twenty-five male Wistar rats, were divided into 5 groups: 1) normal (N); 2) Hyperlipidemia (HL); 3) Hyperlipidemia with fiber 1.04 g/rat/day (HL1); 4) Hyperlipidemia with fiber 2.07 g/rat/day (HL2), and 5) Hyperlipidemia with fiber 3.11 g/rat/day (HL3). The rats were considered hyperlipidemia, if their plasma triglyceride levels were > 70.79 mg/dL. High fat and fructose diet contain high fructose and trans-fat, while intervention diet for T1, T2, and T3 contain a total fiber of 6.88%, 13.77% and 20.65%, respectively. Serum IL-10 analysis using ELISA method was done before and after intervention, and IL-10 gene expression was analyzed using qPCR method after intervention. High trans-fat and fructose diet decrease IL-10 levels, while high-fiber diet can significantly increase the gene expression and levels of IL-10 in hyperlipidemia rats (p<0.05). High-fiber diet can improve inflammation through increase the gene expression and levels of IL-10.