Molecular genetic markers associated with salt stress in local and hybrid corn

Corn is the second most important cereal crop after rice. Salinity, drought, alkalinity, temperature, and
mineral toxicity is one of major limiting factors on plant production include corn. This research objective
was to study molecular marker RAPD associated with salt tolerance on local Madura (Var. Elos and var.
Duko) and hybrid corn (var. Anoman and var. Sukmaraga). Those corns were planted by wick system
hydroponic for 3 weeks. Media used as MS + NaCl (0, 100, and 200mM). DNA was isolated by nucleon
phytopure protocol then amplified using PCR. The amplified fragments profile was defined by the
presence (1) and absence (0) at particular positions on the gel. Cluster analysis and dendrogram had
been done by Unweighted Pair-Group Method Arithmetic (UPGMA) used Multi-Variate Statistical
Package (MVSP). The results were show that the corns which had tolerance to salt stress were in the
same forks or in one group. Its found 120 scorable amplified DNA fragments ranging in size from 139 to
1772 base pairs. Seven positive primers were found to be associated with salt stress, while six negative
primers found.