The Association of Single Nucleotide Polymorphism (SNP) g.281G > A of CAST Gene with Meat Quality of Boerka Goat

Calpastatin gene has been known as a candidate gene for meat quality in cattle, sheep, and chicken. The purpose of this study was to identify CAST gene polymorphisms and its association with meat traits in Boerka goat. The data of pH, cooking loss (CL), warner-bratzler shear force (WBSF), water holding capacity (WHC), cholesterol, water, ash, fat, and protein contains were recorded. Sequencing of 21 samples revealed five polymorphisms of CAST gene in intron 12 within Boerka goat, namely g.146C > A, g.224A > G, g.281G > A, g.737C > T, and g.431G > A. Only single nucleotide polymorphism (SNP) g.281G > A was used for genotyping. The genotype and allele frequency based on g.281G > A showed 14.29% (GG genotype) and 85.71% (GA genotype) followed with 57% G allele and 43% A allele. The chi-square test showed deviation from HWE (p <0.05) in Boerka goat. The SNP g.281G > A revealed having significantly effect to CL. The GA animals had lower CL percentage compared to the GG animals. In conclusion, the SNP selected may be used for identify meat having low CL in Boerka goat.