Comparative safety and efficacy of two bivalent vaccines containing Newcastle disease LaSota and avian influenza H9N2 Sidrap isolate formulated with different oil adjuvants

Background and Aim: Newcastle disease (ND) and avian influenza (AI) are two devastating diseases of poultry, which
cause great economic losses to the poultry industry and disrupt food security in our country. The use of ND-AI inactive
bivalent vaccine is very effective and economical to prevent and control ND and AI disease. Bivalent ND LaSota-AI H9N2
vaccine is not yet available in Indonesia. The inactivated vaccines used in poultry industry often require oil adjuvant to elicit
a sufficient immune response. This study aimed to develop the bivalent inactive vaccines containing ND LaSota and AI
H9N2 Sidrap isolate which are local isolates as poultry vaccine candidates, and formulated with two different commercial
adjuvants, then compared.
Materials and Methods: Two vaccines bivalent were prepared by emulsifying inactivated Newcastle disease virus (LaSota
strain) and AI H9N2 Sidrap isolate viruses with Marcol white mineral oil and Montanide ISA70 adjuvants. Both of bivalent
vaccines were tested for safety (physical and histopathological at the injection site) and efficacy in specific-pathogen-free
chickens. Parameters used for the evaluation of the efficacy were immunogenicity by hemagglutination inhibition and
protection percentage.
Results: Both bivalent vaccines are safe to use. Post-vaccination (PV) immune response was observed using a
hemagglutination inhibition test at 2, 3, 4, 5, 6, 7, and 8 weeks of PV. The bivalent vaccine B gives a better immune response
to ND at 2, 3, and 4 weeks of PV (p<0.05) compared to the bivalent vaccine A, but in 5, 6, 7, and 8 weeks, the PV does
not show differences in the immune response. The immune response to AI H9N2 showed differences at weeks 2 and 3 PV
(p<0.05) with the bivalent vaccine B indicated higher immunity. A single immunization with both bivalent vaccines induces
100% protection in chickens that have been vaccinated against the deadly challenge with the virulent ND virus.
Conclusion: Both of bivalent vaccines are safe to use and provide good efficacy against virulent ND viruses, but bivalent
vaccine B (with Montanide ISA70 adjuvant) shows better immune response than bivalent vaccine A (Marcol white mineral
oil adjuvant).