Molecular phylogeny of orchids mycorrhiza isolated from native tropical orchids in Indonesia

Aims: Mycorrhiza has an important role as a biocontrol agent. Its association with Phalaenopsis amabilis was
molecularly identified through rDNA-ITS sequence analysis. The aims of the study were to identify molecular of orchids
mycorrhiza isolate from native tropical orchids in Indonesia, conducted as one of native orchid conservation efforts in
Indonesia.
Methodology and results: One group of Ceratobasidium were isolated from the root of orchid plant in Yogyakarta
based on morphological and microscopical analysis. The results of molecular analysis showed 600-750 bp of DNA
products located on the ITS1-5.8S-ITS4 region. The sequenced products showed insertion and substitution occurances,
which may result in strain diversity and possible variation. Reconstruction of phylogenetic trees using Maximum
Parsimony and Bootstrap-1000 approach showed showed the Indonesian isolate is at the basal clade and already far
apart from the other isolates.
Conclusion, significance and impact of study: Isolate Ceratobasidium from Yogyakarta, Indonesia successfully
isolated based on identification of rDNA-ITS sequences. Results of this study were expected to become the basic
information in an effort of native orchid cultivation and protection against infectious diseases in Indonesia. The study was
the first to report regarding Ceratobasidium isolated from native tropical orchids in Indonesia.