Collagen properties of Indonesian local sheepskin isolated using acid and enzymatic methods

Putri, Dita Prameswari Trenggono and Pangestika, Vernanda Widya and Ilyas, Hanifan and Abidin, Mohammad Zainal and Fitriyanto, Nanung Agus and Erwanto, Yuny (2024) Collagen properties of Indonesian local sheepskin isolated using acid and enzymatic methods. Journal of Advanced Veterinary and Animal Research, 11 (3). 722 – 728. ISSN 23117710

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Abstract

Objectives: This study aimed to investigate the physical properties of Indonesian local sheep skin collagen extracted by acid and enzymatic methods. Materials and Methods: Collagen was isolated from Pure Breed Garut Sheep (Ovis aries sp.) skin, 1.5 years old. The skins were obtained from a local slaughterhouse in Cirebon, Indonesia. The solvents used were CH3COOH and three different enzymes: neutrase, alcalase, and bromelain. Results: The highest yields of extracted collagen were bromelain-soluble collagen (BSC), which reached 37.07. The range of Ph values for all samples started from 4.01 to 4.76. The viscosity values (cP) of acid-soluble collagen (ASC), neutrase-soluble collagen (NSC), alcalase-soluble collagen (LSC), and BSC were 3.42, 3.90, 3.45, and 3.12, respectively. Regarding SDS-PAGE analyses, Garut sheepskin collagen is categorized as collagen type I, which has a molecular weight of about 140.99 to 148.74 kDa for α1 and around 110 to 111.86 kDa for α2. The results of FTIR and DSC analyses for all samples show the same motif with commercial collagen motifs based on the literature. Conclusion: Garut sheep skin has the potential to be an alternative raw material source for producing collagen. Collagen extracted using a combination of CH3COOH and bromelain enzyme showed the most desirable results in almost every characteristic. © The authors.

Item Type: Article
Additional Information: Cited by: 0; All Open Access, Gold Open Access, Green Open Access
Uncontrolled Keywords: Enzymatic method; Acid method; Collagen; Characteristics; Garut Sheep
Subjects: S Agriculture > SF Animal culture
Divisions: Faculty of Animal Sciences > Department of Animal Products Technology
Depositing User: Wirasto Wirasto
Date Deposited: 23 May 2025 06:43
Last Modified: 23 May 2025 06:43
URI: https://ir.lib.ugm.ac.id/id/eprint/18269

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