Anthelmintic Activity of Red Macroalgae Acrocystis sp. and Acanthophora sp. Etanolic Extract Against Haemonchus contortus in Sheep In Vitro

This study aimed to evaluate the anthelmintic activity of ethanolic extracts from red macroalgae Acrocystis sp. and Acanthophora sp. against the nematode Haemonchus contortus found in sheep, in vitro. The adult worm motility test was conducted in a factorial completely randomized design with two factors: the type of macroalgae as the first factor and the time of observation as the second factor. A total of 100 adult female H. contortus worms were randomly divided into four treatments with five replicates each. Each replicate consisted of 5 worms placed in a petri dish containing 5 mL of 0.9% sodium chloride solution (C: negative control), 1 mg/mL ethanolic extract of Acrocystis sp. (AR), 1 mg/mL ethanolic extract of Acanthophora sp. (AC), and 0.5 mg/mL albendazole (ALB: positive control). Sodium chloride 0.9% was used as the solvent for AR, AC, and ALB. The results demonstrated that, after 24 hour, AC exhibited anthelmintic activity by inhibiting the motility of H. contortus (P<0.05), to a remaining 4.00%, compared to AR (44.00%), although it could not compete with albendazole (P<0.05). Accordingly, the ethanolic extract of red macroalgae Acanthophora sp. holds potential for further investigation as an anthelmintic agent for ruminant livestock.