Chemometrics Assisted LC-HRMS Non-Targeted Metabolomics for Discrimination of Beef, Chicken, and Wild Boar Meats

Windarsih, A. and Rohman, A. and Khasanah, Y. and Erwanto, Y. and Abu Bakar, N.K. (2024) Chemometrics Assisted LC-HRMS Non-Targeted Metabolomics for Discrimination of Beef, Chicken, and Wild Boar Meats. Tropical Animal Science Journal, 47 (3). 381 – 391. ISSN 2615787X

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Abstract

Meat authentication is very important to avoid adulteration, substitution, and mislabeling of meats and meat-based products to protect consumers by ensuring quality, safety, and halal status. This research aimed to employ metabolomics approach using liquid chromatography-high resolution mass spectrometry (LC-HRMS) to identify metabolites of beef (BM), chicken meat (CM), and wild boar meat (WBM) as well as to identify the discriminating metabolites of BM-WBM and CM-WBM. The chemometrics of principal component analysis (PCA) and partial least square-discriminant analysis (PLS-DA) were used to differentiate BM, CM, and WBM. The orthogonal projection to latent structures-discriminant analysis (OPLS-DA) was used to discriminate and identify discriminating metabolites of BM-WBM and CM-WBM through the variable importance for projections (VIP) value analysis (VIP>1.50, p<0.05). The heatmap plot showed the distribution of discriminating metabolites in BM, CM, and WBM samples. The results of this study suggested that untargeted LC-HRMS successfully identified metabolites in meats. In addition, chemometrics could be used to discriminate between BM, CM, and WBM clearly. In summary, the combination of LC-HRMS and chemometrics is promising for the authentication of meats to ensure the quality as well as halal status of meats. Copyright © 2024 by Authors, published by Tropical Animal Science Journal. This is an open-access article distributed under the CC BY-SA 4.0 License (https://creativecommons.org/licenses/by/4.0/).

Item Type: Article
Additional Information: Cited by: 1; All Open Access, Gold Open Access
Uncontrolled Keywords: chemometrics; halal authentication; LC-HRMS; metabolomics
Subjects: S Agriculture > SF Animal culture
Divisions: Faculty of Animal Sciences > Department of Animal Products Technology
Depositing User: Wirasto Wirasto
Date Deposited: 27 May 2025 07:53
Last Modified: 27 May 2025 07:53
URI: https://ir.lib.ugm.ac.id/id/eprint/18677

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