Molecular Identification, Chemical Composition, and In Vitro Anthelmintic Activity of Sargassum duplicatum Against Haemonchus contortus

Sakti, A.A. and Kustantinah, Kustantinah and Sofyan, A. and Nurcahyo, R.W. and Fidriyanto, R. and Kusnadi, H. and Prasetyo, A. and Putnarubun, C. and Permadi, S. and Pramono, Pramono and Hartati, L. and Hudaifa, I. and Suwignyo, B. (2024) Molecular Identification, Chemical Composition, and In Vitro Anthelmintic Activity of Sargassum duplicatum Against Haemonchus contortus. Tropical Animal Science Journal, 47 (2). 188 – 196. ISSN 2615787X

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Abstract

The resistance of the Haemonchus contortus, a parasite causing severe anemia in ruminants, to commercial anthelmintics emphasizes the need for alternative bio-anthelmintics. This study aimed to identify the molecular, chemical composition, and in vitro anthelmintic activity of Sargassum duplicatum against H. contortus. Molecular identification employed the Chelex method, with DNA diversity and phylogeny assessed using maximum likelihood in IQ-tree. The analyzed chemical composition included proximate, total flavonoid, and total phenols. Adult worm motility test (AWMT) and egg hatch inhibiting test (EHIT) were conducted at concentrations of 2, 4, and 6 mg/mL of S. duplicatum ethanolic extract. AWMT followed a completely randomized factorial design (5 replications, each with 5 worms), while EHIT used a completely randomized one-way design (5 replications, each with H. contortus egg batches from 3 adult female worms). The cox1 gene sequence revealed the Sargassum sample as S. duplicatum (KP101270.1) with 99.83 similarity. The results indicated that the identified concentrations of S. duplicatum ethanolic extract, and the observation time significantly influenced motility and egg hatchability (p<0.05). Both factors exhibited a significant interaction (p<0.05). Concentrations of 4-6 mg/mL reduced worm motility by up to 50 (LD50) within 6-8 hours, while concentrations of 2-6 mg/mL inhibited egg hatchability by more than 87 during 24 hours of incubation. In conclusion, S. duplicatum holds significant potential as a bio-anthelmintic agent. © (2024), by Authors, published by Tropical Animal Science Journal.

Item Type: Article
Additional Information: Cited by: 1; All Open Access, Gold Open Access
Uncontrolled Keywords: anthelmintic; Haemonchus contortus; Sargassum duplicatum; sheep
Subjects: S Agriculture > SF Animal culture
Divisions: Faculty of Animal Sciences > Department of Animal Nutrition and Feed Science
Depositing User: Wirasto Wirasto
Date Deposited: 03 Jun 2025 04:28
Last Modified: 03 Jun 2025 04:28
URI: https://ir.lib.ugm.ac.id/id/eprint/18728

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