Effect of Yellowfin Tuna Bone Derived Hydroxyapatite on Hela and Vero Cell Line Proliferation

This study aimed to evaluate the proliferation of Vero and Hela cells after exposed to yellowfin tuna bone derived hydroxyapatite (THA). Vero and Hela cells were cultured in M199 medium and RPMI respectively, supplemented with 10 FBS, 2 Penicillin and Streptomycin, and 0.5 fungizone. The cells were exposed to various concentrations (12.5 to 100 µg/ml) of THA for 24 and 48 hours. The proliferation of the cells was examined using MTT assay. The results showed that the proliferation percentage of Vero and Hela cells was more than 100 in all treatment groups. ANOVA showed no statistically difference in the optical density (OD) values among the groups of Vero cells exposed to THA for 24 and 48 hours (p>0.05). However, OD values of the Vero cells exposed to 25, 50 and 100 µg/ml THA after 48 hours exposure were significantly higher compared to the control group (p<0.05). ANOVA showed statistically difference in the OD values among the Hela cells (p<0.05) after 24 and 48 hours exposure to various concentration of THA. In conclusion, Vero and Hela cells enhance their proliferation after exposed to THA, suggesting the potency of THA to be used as a biomaterial. © 2020. All Rights Reserved.