Expression of Non-Metastatic Protein-23 And Metastatic Associated Protein-1 as a Molecular Target Therapy of an Oral Malignant Burkitt’s Lymphoma Induced by Oligonucleotide P27 Sense

Burkitt’s lymphoma (BL) is an uncommon type of Non-Hodgkin lymphoma (NHL). It is a highly aggressive type of B-cell lymphoma and the most common childhood cancer. Treatment for this cancer is still limited. However, a new strategy for refractory tumor, gene therapy is watched with keen interest.The aim of study was to investigate the expression of non-metastatic 23 (nm-23) and metastatic associated protein-1 (MTA-1) of an oral malignant Burkitt’s lymphoma induced by oligonucleotide p27 sense (p27 S). The true experimental study with post-test only control group design was confirmed in this study. For detection of nm-23, MTA-1, p27Kip-1 and α- tubulin protein expressions were carried-out by Western blotting analysis. Induction of apoptosis was analyzed by double staining using acridine orange-ethidium bromide (AO-EB). Results revealed the expression of nm-23 and p27Kip-1 protein was markedly increased in cell transfected with p27 sense. Contrally, down regulation of MTA-1 protein was detected in these cell transfectant. Apoptosis induction was significantly elevated in Raji-p27 S compared with that of p27 AS. In conclusion, oligonucleotide p27 S has a strong antitumor activity in oral malignant Burkitt’s lymphoma through increasing the apoptosis activity, expression of nm-23 and p27Kip-1 protein induction, and suppressing the expression of MTA-1 protein targeting this molecule could represent a promising new therapeutic approach for this type of cancer. © 2020