The Effect Protease Inhibitor on the Composition of Molecular Weight of Abalone (Haliotis varia Linnaeus) Fractions

Arginine is one of the important components of the latest technology in making the desensitizing materials in the field of dentistry. Abalone is one of the marine biotas containing a high level of arginine. The extraction process and fractionation of Abalone (H. varia Linnaeus) to obtain the gel of desensitizing agent require a certain procedure to obtain the molecular weight as expected. This research aims to observe the effects of the addition of the protease inhibitor on the composition of the molecular weight of the abalone fractions as a preliminary study for being used as dental material. Furthermore, a preparative chromatography procedure was conducted using the qualitative thin layer chromatography (TLC). The fractions results were dried and dissolved into the solution, centrifuged at stages, and then the supernatant was taken. Subsequently, the composition of molecular weight was observed with 12% SDS-PAGE electrophoresis gel. Then, it was analyzed using Image J software to measure the density of the band of electrophoresis gel and determine a molecular weight composition. The data were tested statistically using One-way ANOVA and continued with post hoc Tukey's HSD. The results of the analysis showed a significant difference in the composition of molecular weight (p <0.05). Post hoc analysis showed a significant difference (p <0.05) between fractions groups. According to the results of the present study, it can be concluded from this study that protease inhibitor has an effect on the molecular weight composition of heavy, medium and light levels.