Positive NS1 Antigen in Non-Dengue Virus Infection Serum: Possible Reasons for the Discrepancy with DENV PCR Results

Sunari, I. Gusti Agung Ayu Eka Putri and Aryati, Aryati and Hakim, Faradila Khoirun Nisa and Tanzilia, May Fanny and Parwati, Ida and Yaswir, Rismawati and Mulyono, Budi (2024) Positive NS1 Antigen in Non-Dengue Virus Infection Serum: Possible Reasons for the Discrepancy with DENV PCR Results. Pharmacognosy Journal, 16 (4). pp. 923-926. ISSN 09753575

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Abstract

Background and Objective: A specific examination is required to distinguish between DVI and viral, bacterial, and parasitic illnesses because their clinical manifestations are nearly identical. Leukopenia and lymphocytosis are examples of non-specific tests that might be used to get a diagnosis. Non-structural protein 1 (NS1) antigen, anti-DENV antibody, or DENV-specific nucleic acid detection are more specific assays. Methods: Virus isolation or molecular analysis of the detection of DENV nucleic acid ribonucleic acid (RNA) using RT-PCR was used to make the conclusive diagnosis of DVI. The sensitivity of the DENV RT-PCR method ranges from 28.8 to 99. NS1 antigen is used as an initial diagnostic option in primary health care because it has a high specificity value (100). Researchers want to analyze the positivity in non-DVI samples that have been confirmed by real-time RT-PCR examination with semi-quantitative NS1 antigen examination. Patient population aged 1-65 years with acute fever <5 days. A total of 130 samples of non-DVI confirmed patients by RT-PCR were examined for NS1Antigen ELISA. Results: With a proportion of 3.08 of the total sample, the results showed that 4 NS1Antigen ELISA samples were positive. A negative DENV RT-PCR result could indicate either a true negative or a false negative. Conclusion: The type of PCR technology, the primer used, the existence or absence of a DENV mutation, the DENV serotype, and the presence of mismatched nucleotides can all affect variations in DENV PCR sensitivity. © 2024 Phcogj.Com.

Item Type: Article
Additional Information: Cited by: 0; All Open Access, Gold Open Access
Uncontrolled Keywords: nonstructural protein 1; virus RNA; adolescent; adult; aged; Article; child; dengue; Dengue virus; enzyme linked immunosorbent assay; false negative result; female; fever; human; major clinical study; male; nonhuman; optical density; real time reverse transcription polymerase chain reaction; school child; sensitivity and specificity; virus detection; virus isolation
Subjects: R Medicine > RZ Other systems of medicine
Divisions: Faculty of Medicine, Public Health and Nursing > Non Surgical Divisions
Depositing User: Ani PURWANDARI
Date Deposited: 08 Jul 2025 06:09
Last Modified: 08 Jul 2025 06:09
URI: https://ir.lib.ugm.ac.id/id/eprint/19394

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