Mixed Culture with Monoraphidium sp. for the Increase of Metabolites Production in Glagah Strain Consortium

The feasibility of microalgae-derived products became a major issue on an industrial scale. Mixed culture is one of the improvements that can be made. The mixed culture of Glagah Strain Consortium and Monoraphidium sp. was investigated in terms of biomass, lipid, protein, and carbohydrates production, as well as productivity. Glagah Strain Consortium is a local consortium strain composed primarily of Cylindrospermopsis sp., Cyclotella sp., Corethron sp., Golenkinia sp., Chlamydomonas sp., and Syracosphaera sp., native to Glagah Lagoon in Yogyakarta, Indonesia. The mixed culture of Glagah Strain Consortium and Monoraphidium sp. was compared to the monocultures of Glagah Strain Consortium only and Monoraphidium sp. only. It is then found that mixed cultures can increase lipid productivity up to 0.1808±0.010 g/L/day, which is greater than monocultures. Mixing the culture with Monoraphidium sp. was shown to increase production and productivity of lipid, biomass, protein, and carbohydrates, though not as much as Monoraphidium sp. monoculture. Further research is needed to understand the mechanisms in the mixed culture and to increase the culture’s production and productivity. © (2025), (Akademi Sains Malaysia). All rights reserved.