In vitro digestibility and ruminal fermentation profile of ruminantdiet in response to substitution of mixture feedstuff protected

Excess degradation of high protein feed in the rumen can reduce the efficiency of post-rumen nutrientutilization. The protected feed is expected to increase the protein absorption in the intestine. Meanwhile,supplementation of oil or fat in the ration has a negative effect due to biohydrogenation of fat in the rumen.Protection of fat is expected to reduce these negative effects in the rumen. This in vitro study was conducted todetermine the rumen fermentation parameters of a mixture of soybean meal, saponified crude palm oil (CPO)and Agromix mineral premix which was protected with formaldehyde. The crude palm oil used is protectedby the saponification method (saponification) and mixed with soybean meal and Agromix mineral premix.The ratio of saponified CPO with soybean meal is 1: 4 with the addition of Agromix mineral premix 0.3 ofthe total production of protective feed. The mixture of the three ingredients is then protected with formaldehyde(formalin 0.8). Independent sample T-Test was used to compare 2 treatments (CTL/ control = commercialrations; 7.6PF = commercial rations with 7.6 protected feed substitution). All treatments were incubated for48 and 96 hours according to the 2-stage in vitro technique. Protected feed substitution can increase the efficiency of ruminant feed that contains high protein and reduce thenegative effect of fat on rumen microbes. This is evidenced by the increase in IVDMD, IVOMD, and IVCPD ofration in post-rumen. © 2021 Elsevier B.V., All rights reserved.