Effect of methionine supplementation on intestinal morphology in broilers infected with aflatoxicosis B1

This study aimed to evaluate methionine supplementation to ameliorate toxicity of aflatoxin B<inf>1</inf> (AFB<inf>1</inf>) on intestinal morphology in broilers. A total of 320 broilers were randomly allocated to 5 treatments with 8 replicates, 8 birds per replicate. The dietary treatments were The dietary treatments (AF = Aflatoxin B<inf>1)</inf> were as follows: CTL: control (basal diet, without AF), AF100 (100 µg/kg of AF), AF500 (500 µg/kg of AF), AFM100 (100 µg/kg AF+ DL-methionine 0.3 g/kg), AFM500 (500 µg/kg AF+ DL-methionine 3 g/kg). The results showed that AFB<inf>1</inf> 500 µg/kg decreased villus height and width(p<0.05). Both 100 and 500 µg/kg increased crypt depth (p<0.01), also decreased villus:crypt (V:C) ratio. Supplementation of methionine ameliorated villus height, villus width, and V:C ratio of broilers fed AFB<inf>1</inf> contaminated diet. However, it did not affect crypt depth. In conclusion, the addition of methionine reduced the adverse effect of aflatoxicosis B<inf>1</inf> on intestinal growth in broilers. © 2020 Elsevier B.V., All rights reserved.