Hanafi, Muchtar and Muhammad, Faizal and Ramadhani, Amilia and Nurlaela, Nurlaela and Sofro, Zaenal Muttaqien and Partadiredja, Ginus (2023) Subchronic Administration of High-dose Monosodium Glutamate Causes Spatial Memory Dysfunction and Structural Deficits of Rat Hippocampus. Malaysian Journal of Medicine and Health Sciences, 19 (4). pp. 159-165. ISSN 16758544
Subchronic Administration of High-dose Monosodium Glutamate.pdf - Published Version
Restricted to Registered users only
Download (1MB) | Request a copy
Abstract
Introduction: The toxicity of high concentration monosodium glutamate (MSG) has become a controversial issue because of its inconsistent results in human and animal studies. This present study aims to evaluate the effect of subchronic high-doses oral administration of MSG on spatial memory performance and hippocampal pyramidal cells number. Methods: This study involved twenty-eight male Wistar rats, which were divided into a control group of NaCl 0.9 and three intervention groups of MSG 1.0 mg/g bodyweight (M1), 2.0 mg/g bodyweight (M2), and 4.0 mg/g bodyweight (M3) for 30 days. Statistical analysis used a One-way ANOVA test. Results: The result showed significant differences in spatial memory on the Morris Water Maze (MWM) test, including path length (p = 0.020) and escape latency (p = 0.011) according to general linear model repeated measurement analysis. The mean difference of estimated hippocampal pyramidal cells total number among the groups showed volume (p = 0.001), numerical density (p = 0.590), and cells number (p = 0.004). Furthermore, Post-Hoc analysis in both spatial memory and hippocampal pyramidal cells showed that the increasing MSG dose from 1.0 to 4.0 mg/g bodyweight led to a decrease in the results of spatial memory performance on the MWM test and a decrease in hippocampal cells. Conclusion: The present study has provided novel quantitative data that subchronic administration of high-dose MSG caused deleterious effects on the spatial memory function and the volume and number of hippocampal pyramidal cells. Malaysian Journal of Medicine and Health Sciences (2023) 19(4):159-165. doi:10.47836/mjmhs19.4.24. © 2023 UPM Press. All rights reserved.
Item Type: | Article |
---|---|
Additional Information: | Cited by: 0; All Open Access, Bronze Open Access |
Uncontrolled Keywords: | Cognitive, Hippocampus, Monosodium glutamate, Spatial memory, Stereology |
Subjects: | R Medicine > RC Internal medicine > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry |
Divisions: | Faculty of Medicine, Public Health and Nursing > Biomedical Sciences |
Depositing User: | Annisa Fitria Nur Azizah Annisa Fitria Nur Azizah |
Date Deposited: | 29 May 2024 08:41 |
Last Modified: | 29 May 2024 08:41 |
URI: | https://ir.lib.ugm.ac.id/id/eprint/2368 |