Subchronic Administration of High-dose Monosodium Glutamate Causes Spatial Memory Dysfunction and Structural Deficits of Rat Hippocampus

Hanafi, Muchtar and Muhammad, Faizal and Ramadhani, Amilia and Nurlaela, Nurlaela and Sofro, Zaenal Muttaqien and Partadiredja, Ginus (2023) Subchronic Administration of High-dose Monosodium Glutamate Causes Spatial Memory Dysfunction and Structural Deficits of Rat Hippocampus. Malaysian Journal of Medicine and Health Sciences, 19 (4). pp. 159-165. ISSN 16758544

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Abstract

Introduction: The toxicity of high concentration monosodium glutamate (MSG) has become a controversial issue because of its inconsistent results in human and animal studies. This present study aims to evaluate the effect of subchronic high-doses oral administration of MSG on spatial memory performance and hippocampal pyramidal cells number. Methods: This study involved twenty-eight male Wistar rats, which were divided into a control group of NaCl 0.9 and three intervention groups of MSG 1.0 mg/g bodyweight (M1), 2.0 mg/g bodyweight (M2), and 4.0 mg/g bodyweight (M3) for 30 days. Statistical analysis used a One-way ANOVA test. Results: The result showed significant differences in spatial memory on the Morris Water Maze (MWM) test, including path length (p = 0.020) and escape latency (p = 0.011) according to general linear model repeated measurement analysis. The mean difference of estimated hippocampal pyramidal cells total number among the groups showed volume (p = 0.001), numerical density (p = 0.590), and cells number (p = 0.004). Furthermore, Post-Hoc analysis in both spatial memory and hippocampal pyramidal cells showed that the increasing MSG dose from 1.0 to 4.0 mg/g bodyweight led to a decrease in the results of spatial memory performance on the MWM test and a decrease in hippocampal cells. Conclusion: The present study has provided novel quantitative data that subchronic administration of high-dose MSG caused deleterious effects on the spatial memory function and the volume and number of hippocampal pyramidal cells. Malaysian Journal of Medicine and Health Sciences (2023) 19(4):159-165. doi:10.47836/mjmhs19.4.24. © 2023 UPM Press. All rights reserved.

Item Type: Article
Additional Information: Cited by: 0; All Open Access, Bronze Open Access
Uncontrolled Keywords: Cognitive, Hippocampus, Monosodium glutamate, Spatial memory, Stereology
Subjects: R Medicine > RC Internal medicine > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
Divisions: Faculty of Medicine, Public Health and Nursing > Biomedical Sciences
Depositing User: Annisa Fitria Nur Azizah Annisa Fitria Nur Azizah
Date Deposited: 29 May 2024 08:41
Last Modified: 29 May 2024 08:41
URI: https://ir.lib.ugm.ac.id/id/eprint/2368

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