Formulation of Alginate Beads to Encapsulated Kaffir Lime Callus

Our previous study revealed that callus induced from kaffir lime seeds have an ability to produce bioactive compounds for traditional medicine. In order to scale up the production, it requires an in vitro storage method to provide a continuous supply of callus. Encapsulation is one of the in vitro storage methods which can maintain the viability of callus and stability of bioactive compounds. Alginate capsule formation depends on sodium alginate ( NaCd l .( ),,!and calcium chloride dehydrate (CaCb.2HsO) concentrations, as well as the maturation time. While maintaining callus viability, growth medium addition to the capsule is required. Therefore, objective of this study is to investigated an efficient protocol by determining the concentration of NaCTHrOs, CaCh.2H0. nourish medium and maturation time to produce springy texture and consistent shape of capsule. This study using 5 concentrations of alginate: 1.5.2, 3 and 4 immersed at 4 concentrations of CaC'h.2IhO, which were 0.1; 0.15; 0.2 and 0.4 M for 60 minutes. The capsules were analyzed by scoring capsule formation ability; consistency and texture of the capsules springy or sticky. The best formula is then nourished by MS medium with the concentration 1 , Vi,and 14 (v/v). The results showed that 4 alginate added with 14 dosage of MS medium and immersed in 0.2 M CaCh.21 hO for 30 minutes w as capable to produce capsules with best texture and consistency. © 2020 Elsevier B.V., All rights reserved.