Effect Analysis of Prolactin (PRL) Gene Polymorphisms on Chicken Egg Productivity (Gallus gallus domesticus) BC1 from Crossbreeding between Pelung and Layer Chicken

Prolactin (PRL) Gene in chicken was closely related to broodiness and brooding behavior that located at chromosome 2. This research was conducted to detect insertion/deletion (in/del) 24-bp polymorphism in the prolactin promoter gene and its association with the BC<inf>1</inf> hybrid chicken egg productivity. Procedures of this research conducted are chicken maintenance, egg collection for 23 weeks of production, eggs proximate quality (water, ash, protein, carbohydrate, fat, and fiber percentage), DNA isolation, PRL gene amplification, and visualizing DNA bands on PRL promoters. The results showed the average number of cumulative eggs of BC<inf>1</inf> chicken for 23 weeks was 42.9 eggs, lower than layer (104.34 eggs), and higher than pelung (30.17 eggs). Analysis using the pearson correlation test shows the frequency of insertion-deletion polymorphism alleles on the PRL promoter has a signification value of 0.521 and correlation value 0,684, so it is concluded that there is no correlation (P>0.05). Proximate test results of BC<inf>1</inf> whole raw egg chicken has characteristics of low fat (3.2) and high carbohydrate (9.1) compared to commercial egg and has a calorie content of 98.37/100 g of the sample. © 2021 Elsevier B.V., All rights reserved.