Immuno-enhancement activity of meniran (Phyllanthus niruri L.) and temu mangga rhizome (Curcuma mangga Val.) combination extract in cyclophosphamide-induced immunodeficient mice

Immunomodulators play a crucial role in maintaining overall health and enhancing the body's defense against various diseases. In Indonesian traditional medicinal practices, meniran (Phyllanthus niruri L.) and temu mangga rhizome (Curcuma mangga Val) are well-regarded for their health benefits and therapeutic properties. Notably, meniran is utilized in jamu, an ancient Indonesian herbal tonic with widespread use in traditional healthcare. This study aimed to formulate a synergistic herbal blend by combining ethanolic extracts of meniran and temu mangga rhizome, assessing their efficacy against Cyclophosphamide (CYP)-induced immunosuppression in a murine model and elucidating potential mechanisms for immune restoration. We conducted quantitative and qualitative investigations of rutin and demethoxycurcumin composition in the combination extracts using high-performance thin-layer chromatography (HPTLC). An in vivo investigation was performed using a CYP-induced immunosuppressed murine model to assess the immunomodulatory effects of the combination extracts. The immunostimulant effect was investigated by analyzing hematology, macrophage activity (Nitric Oxide secretion, phagocytic capacity and index), lymphocyte proliferation (MTT assay), cytokine levels (IL-6 and TNF-α by ELISA), and analysis of CD4+ and CD8+ T cell populations using flow cytometry. Results demonstrated that the combined extracts effectively mitigated the immunosuppressive effects of CYP, significantly enhancing various immune indicators, including red blood cell count, hemoglobin levels, macrophage activity, lymphocyte proliferation, and cytokine serum levels. However, negligible effects were observed on erythrocyte indexes compared to the CYP-induced group. In conclusion, the combination of meniran (Phyllanthus niruri L.) and temu mangga rhizome (Curcuma mangga Val) substantially enhances various aspects of immune function, such as red blood cell count, hemoglobin levels, and macrophage activity, suggesting promising potential for immunomodulation in therapeutic contexts. © 2024 Elsevier B.V.