Integrating Milk Protein Hydrolysate and Plasma-Activated Water as Alternative Nitrogen Inputs for Growth, Nutrition, and Postharvest Quality of Hydroponic Cos Lettuce Under Low Nutrient Supply

The application of plasma-activated water and biostimulants offers a sustainable approach to supporting plant growth under reduced-nutrient conditions by supplying bioavailable nitrogen. This study investigated the growth and postharvest performance of hydroponically grown cos lettuce (Lactuca sativa L.) supplied with three Hoagland-based nutrient treatments: half-strength solution prepared with tap water (HS), half-strength solution with plasma-activated water (HS+PAW), and half-strength solution with plasma-activated water containing 1 mL L¹ milk protein hydrolysate (HS+PAW+MPH). Plants treated with PAW, particularly those in the HS+PAW+MPH, exhibited increases in growth, biomass accumulation, and mineral composition, with reduced nitrate content compared to controls. At harvest, lettuce under HS+PAW+MPH exhibited nearly double fresh yield and enhanced dry matter, protein, lipid, phenolic, and flavonoid profiles as well as increased antioxidant capacity, indicating improved nitrogen utilization and nutritional quality under reduced nutrient input. Postharvest quality was evaluated by packing samples in polypropylene bags and storing them at 10 ± 1 °C and 95-98 relative humidity for 21 days. The HS+PAW+MPH treatment substantially suppressed respiration and production of ethylene, limited weight loss and color change, and better preserved pigments, bioactive compounds, and antioxidant stability compared to HS and HS+PAW, indicating HS+PAW+MPH as a sustainable nutrient management approach for hydroponic systems. © 2026 by the authors.