Secretome of bovine umbilical vein endothelial cells promote wound healing regeneration on the second degree rat model burn injury

Background: Burn injuries are an alarming indicator of the sensitivity of human tissue when confronted with high
temperatures or chemicals. The current treatment for burn wounds needs to be improved and more extensive in scope.
Significant research advances concerning the therapeutic potential of secretomes over the past two decades have
expanded the range of therapies that utilize secretomes to encompass populations other than stem cells.
Aim: This study details how the secretome extracted from the bovine umbilical vein endothelial cell (BUVEC)
promotes the healing of burn injuries.
Methods: The 48 rats were divided into four groups, namely the control group with povidone-iodine, the 5% BUVECconditioned medium (CM) cream group, the 10% BUVEC-CM cream group, the 15% BUVEC-CM cream group.
Animals induced type II burns under anesthesia. Treatment is carried out topically, two times a day. Every day the
wound was measured. The animals were put to sleep for samples on days 5, 13, 21, and 19. Samples in the form
of skins were soaked in 4% paraformaldehyde and processed with paraffin-embedded for tissue preparations. The
research results were processed using two way ANOVA.
Results: The study showed that on day 5, wound closure occurred, whereas in the povidone-iodine group,
macroscopically, the wound closed faster. Epithelial repair, increased fibroblasts and collagen, and blood vessel
formation greatly increased in the 15% BUVEC-CM group on days 13, 21, and 29.
Conclusion: Taken together, BUVEC secretome promoted fibroblast regeneration, collagen formation,
re-epithelialization, and hair follicle regeneration on the burn injury wound healing.