Short Communication: Polymorphism of collagen type X (COLX) gene and their association with egg production traits and egg weight in Alabio and Mojosari ducks

Kumalawati, Dewi Sari and Maharani, Dyah and Sari, Apriliana P. Z. N. L. and Pratomo, Ganjar H. and Sasongko, Heru and Fathoni, Akhmad and Susanti, Triana (2023) Short Communication: Polymorphism of collagen type X (COLX) gene and their association with egg production traits and egg weight in Alabio and Mojosari ducks. Biodiversitas, 24 (3). 1518 – 1523. ISSN 1412033X

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Abstract

Type X collagen (COLX) is non-fibrillar collagen expressed in the white isthmus of the avian oviduct and plays a role in egg formation. This study aimed to identify COLX gene polymorphism and its association with egg production and egg weight in Alabio (Anas platyrhynchos Borneo) and Mojosari ducks (Anas platyrhynchos Javanicus). Blood samples of 100 female ducks were collected, and their deoxyribonucleic acid (DNA) was extracted. In addition, a 220 bp fragment of COLX gene was amplified using the polymerase chain reaction (PCR) method and sequenced for single nucleotide polymorphism (SNP) identification and genotyping. Data analysis was performed to calculate genotype and allele frequencies, and an independent sample t-test was used to examine the association between genotype and egg production and egg weight of each individual. Three genotypes (CC, TT, and CT) of SNP g.74C/T were detected in both breeds. Allele C (0.77) was dominant in Alabio population; otherwise, allele T (0.81) dominated Mojosari population. The frequency of CC (0.59) was highest in Alabio ducks, followed by CT (0.37) and TT (0.04), while TT (0.65) was highest in Mojosari ducks, followed by CT (0.33) and CC (0.02). Meanwhile, association analysis showed that ducks with CC genotype had significantly higher egg production at 28 and 34 weeks in Alabio than those with CT genotype (p<0.05). These results indicate that COLX is an effective genetic marker for a breeding program to increase egg production in Alabio ducks. © 2023, Society for Indonesian Biodiversity. All rights reserved.

Item Type: Article
Additional Information: Cited by: 0; All Open Access, Gold Open Access
Uncontrolled Keywords: Collagen type X, egg production, egg weight, local duck, single nucleotide polymorphism
Subjects: S Agriculture > SF Animal culture
Divisions: Faculty of Animal Sciences > Department of Animal Nutrition and Feed Science
Depositing User: Uminurida SUCIATI
Date Deposited: 23 Aug 2024 07:43
Last Modified: 23 Aug 2024 07:43
URI: https://ir.lib.ugm.ac.id/id/eprint/3335

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