Fajardo, Roland and Kusuma Astuti, Putri and Bagi, Zoltán and Bodrogi, Lilla and Pintér, Tímea and Skoda, Gabriella and Ohran, Husein and Wanjala, George and Kusza, Szilvia (2023) Continental vs. tropical breed: Immunity comparison under heat stress conditions utilizing qRT-PCR technique. In: BIO Web of Conferences.
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Abstract
Understanding the responses of animals to seasonal heat stress on the genomic level has led to the identification of genes implicated in thermal stress reaction mechanisms. In this study, the relative gene expression of Interleukin-10 (IL-10), an anti-inflammatory cytokine and biomarker for heat stress-mediated immune modulation, was observed during the summer and winter seasons in continental and tropical sheep breeds, namely, Hungarian Indigenous Tsigai and White Dorper, employing quantitative real-time polymerase chain reaction (qRT-PCR). Temperature-humidity indices (THI) were calculated to assess heat stress levels. The results indicate that IL-10 CT-values were significantly higher during the summer, when heat stress prevails, in both sheep breeds compared to winter. While the White Dorper exhibited a higher numerical value for the summer relative gene expression ratio (16.2) compared to the Hungarian Indigenous Tsigai (12.3), no significant differences in CT values were observed between breeds or among sexes. These findings suggest the immune-adaptive characteristics of the two sheep breeds during seasonal heat stress. The variation of IL-10 gene expression levels between the two breeds can be attributed to their geographical origins; the White Dorper emerging from arid subtropical South Africa and the Hungarian Indigenous Tsigai Sheep thriving in the seasonally harsh Carpathian climate for centuries. © The Authors, published by EDP Sciences. All Rights Reserved.
Item Type: | Conference or Workshop Item (Paper) |
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Additional Information: | Cited by: 0; Conference name: 4th International Conference on Smart and Innovative Agriculture, ICoSIA 2023; Conference date: 10 October 2023 through 11 October 2023; Conference code: 195526; All Open Access, Gold Open Access |
Uncontrolled Keywords: | gene expression, heat stress, Hungarian Tsigai, IL-10, White Dorper |
Subjects: | S Agriculture > SF Animal culture |
Divisions: | Faculty of Animal Sciences > Department of Animal Breeding and Reproduction |
Depositing User: | Uminurida SUCIATI |
Date Deposited: | 23 Aug 2024 09:09 |
Last Modified: | 23 Aug 2024 09:09 |
URI: | https://ir.lib.ugm.ac.id/id/eprint/3386 |